Plasmole

Plasmole brings deterministic molecular-biology tools into ChatGPT. Annotate raw DNA (ORF and parts detection), parse GenBank/FASTA files, edit and assemble sequences, design and visualize PCR primers, find restriction sites and predict digests, calculate melting temperature, design CRISPR guides, optimize codons, align two or many sequences, verify a clone against its reference, check a design for problems, export GenBank or FASTA, and fetch public NCBI records by accession, or resolve a gene, plasmid or vector name to one. Plasmid-producing tools render an interactive circular map inline; drag-select a region to design primers against it. Constructs are versioned, so you can compare or restore any earlier snapshot. For research use only.